Isolation and Structural Characterization of the Polypeptide Subunits of Membrane Glycoprotein lib-Illa From Human Platelets
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چکیده
We have previously demonstrated the isolation of platelet membrane glycoprotein lIb-Illa by affinity chromatography with a specific monoclonal antibody.1 We have now separated the polypeptide subunits lIb and lIla of the isolated glycoprotein by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and have compared their structural features. Both llb and lIla contain -.1 5% carbohydrate. but lIla contains a larger percentage of mannose residues. suggesting the presence of high mannose as well as complex N-linked oligosaccharide chains. The amino
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تاریخ انتشار 2005